منابع مشابه
Highly efficient transgenesis in Xenopus tropicalis using I-SceI meganuclease
In this study, we report a highly efficient transgenesis technique for Xenopus tropicalis based on a method described first for Medaka. This simple procedure entails co-injection of meganuclease I-SceI and a transgene construct flanked by two I-SceI sites into fertilized eggs. Approximately 30% of injected embryos express transgenes in a promoter-dependent manner. About 1/3 of such embryos show...
متن کاملEffective RNAi-mediated β2-microglobulin loss of function by transgenesis in Xenopus laevis
To impair MHC class I (class I) function in vivo in the amphibian Xenopus, we developed an effective reverse genetic loss of function approach by combining I-SceI meganuclease-mediated transgenesis with RNAi technology. We generated transgenic outbred X. laevis and isogenetic laevis/gilli cloned lines with stably silenced expression of β2-microglobulin (b2m) critical for class I function. Trans...
متن کاملTransgenesis in C. elegans.
The ability to manipulate the genome of organisms at will is perhaps the single most useful ability for the study of biological systems. Techniques for the generation of transgenics in the nematode Caenorhabditis elegans became available in the late 1980s. Since then, improvements to the original approach have been made to address specific limitations with transgene expression, expand on the re...
متن کاملTransgenesis in nonmurine species.
Although the mouse remains the species of choice for most transgenic experimentation, it may be preferable or even necessary to use alternative species for certain applications. We review the strategies by which transgenic technology has been applied to other animals, specifically, the rat, rabbit, pig, sheep, goat, and cow. Additionally, we outline the potential applications of alternative tra...
متن کاملTransgenesis in farm animals
The production of transgenic animals has many biotechnological applications; however, available techniques still remain inefficient. This review summarizes the contributions of our group to transgenesis in domestic animal. One of the most traditional transgenesis techniques in farm animals is somatic cell nuclear transfer using genetically modified somatic cells. Using this technique, we produc...
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ژورنال
عنوان ژورنال: Biology of the Cell
سال: 2008
ISSN: 0248-4900
DOI: 10.1042/bc20070148